hisat2-build -p thread referenceGenome.fa index
hisat2 -p thread --dta -x index -U SE.fq.gz | samtools sort -@ thread -O bam - > sorted.bam
hisat2 -p thread --dta -x index -1 PE_1.fq.gz -2 PE_2.fq.gz | samtools sort -@ thread -O bam - > sorted.bam